Calculation of molecular number and brightness from fluorescence microscopy image series. The software was published in a 2016 paper <doi:10.1093/bioinformatics/btx434>. The seminal paper for the technique is Digman et al. 2008 <doi:10.1529/biophysj.107.114645>. A review of the technique was published in 2017 <doi:10.1016/j.ymeth.2017.12.001>.
| Version: | 2.1.1 | 
| Depends: | R (≥ 3.1) | 
| Imports: | assertthat, autothresholdr (≥ 1.3.11), BBmisc, checkmate (≥ 1.9.3), detrendr (≥ 0.6.12), dplyr, filesstrings (≥ 3.2), ggplot2, glue (≥ 1.3), ijtiff (≥ 2.2), magrittr (≥ 1.5), purrr, Rcpp (≥ 1.0.1), reshape2, rlang (≥ 0.3.3), stringr (≥ 1.4), utils, viridis, withr (≥ 2.1.0) | 
| LinkingTo: | Rcpp (≥ 1.0.1) | 
| Suggests: | abind, covr, gridExtra, knitr, magick, matrixStats (≥ 0.50), pacman, rmarkdown, spelling, testthat (≥ 2.1), tidyr | 
| Published: | 2025-05-06 | 
| DOI: | 10.32614/CRAN.package.nandb | 
| Author: | Rory Nolan | 
| Maintainer: | Rory Nolan <rorynoolan at gmail.com> | 
| BugReports: | https://github.com/rorynolan/nandb/issues | 
| License: | BSD_3_clause + file LICENSE | 
| URL: | https://rorynolan.github.io/nandb/, https://github.com/rorynolan/nandb | 
| NeedsCompilation: | yes | 
| Language: | en-US | 
| Citation: | nandb citation info | 
| Materials: | README, NEWS | 
| CRAN checks: | nandb results | 
| Reference manual: | nandb.html , nandb.pdf | 
| Vignettes: | Batch mode (source, R code) Brightness timeseries (source, R code) Calculating brightness with single images (source, R code) | 
| Package source: | nandb_2.1.1.tar.gz | 
| Windows binaries: | r-devel: nandb_2.1.1.zip, r-release: nandb_2.1.1.zip, r-oldrel: nandb_2.1.1.zip | 
| macOS binaries: | r-release (arm64): nandb_2.1.1.tgz, r-oldrel (arm64): nandb_2.1.1.tgz, r-release (x86_64): nandb_2.1.1.tgz, r-oldrel (x86_64): nandb_2.1.1.tgz | 
| Old sources: | nandb archive | 
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